식물분포 번역(한국어 원본)1. 서해안 분포한계는 충청남도 태안군( N 36.40)으로 확인되었고, 내륙으로는 충남 논산군(N36°.10＇)과, 나주시 금성산(), 보성군 미력면(N34°.39＇)이었다. 또한 동해안 분포한계는 경주시(N35°.39)였고, 내륙으로는 울진군(N°.＇.. E°.＇)이었다. 부속섬 중에는 울릉도가 분포한계였다.
2. 결실을 맺는 한계지역은 서해안 태안군(N 36.40)과 내륙의 충남 논산군, 보성군, 기장군을 거쳐, 동해안의 경주시(N35°.39), 울진군( N°.＇.. E°.＇)으로 확인되었다. 그러나 지역에 따라 부분적으로 확인되지 않아 재배가 가능한 최적지역은 전라북도 영광군에서 남해안을 포함한 부산시 기장군으로 판단된다.
3. 송악 잎 추출물과 분획물을 DPPH 소거능 측정을 이용하여 항산화활성 검색결과 80% 에탄올 추출물, 에틸아세테이트, 부탄올 분획물에서 높은 radical 소거 활성이 확인되었다. 또한 xanthine oxidase 억제효과는 에틸아세테티즈 분획물에서 확인되었고, superoxide 소거활성은 80% 에탄올 추출물, 디클로로메탄, 에틸아세테이트, 부탄올 분획물에서 각각 확인되었다.
4. RAW 264.7 세포에 LPS로 자극을 주고 추출물과 분획물을 처리하여 확인한 결과, 헥산과 디클로로메탄 분획물에서 NO, iNOS, COX-2, PGE2 생성억제효과가 확인되었다. 그러나 헥산 분획물에서 나타난 생성억제효과는 세포독성에 의한 활성저해 때문에 나타난 것으로 판단된다.
5. 혈액암, 대장암 및 간암세포에 추출물 및 분획물을 처리한 후 MTT assay를 이용하여 암세포 증식억제효과를 확인한 결과 헥산, 디클로로메탄, 에틸아세테이트 분획물에서 세포 증식 억제효과를 확인하였다.
6. HL-60 세포에서 송악의 디클로로메탄 분획물을 처리하여 확인한 결과 세포주기에서 apoptosis 유도에 의해 sub-G1 hypodiploid 세포가 증가하였으며, apoptosis 진행과정 중에 확인되는 apoptotic bodies가 확인되었다. 또한 단백질 발현에서 anti-apoptosis 관련인자인 BCL-xL 생성이 억제되었고 apoptosis 관련인자인 Bax, caspase-3, caspase-9, PARP를 활성화시켰다. 3. 전라남도 송악의 문헌기록과 현지조사 결과 비교 |
식물분포 번역(영어 번역본)1) The distribution limit of the West Coast was Taean-gun, Chungcheong-namdo (N 36.40), and the inland distribution limits were Nonsan-gun, Chungcheong-namdo (N36°.10′), Mt. Geumseongsan of Naju City (), and Miryeok-myeon, Boseong-gun (N34°.39′). Also, the distribution limit of the East Coast was Gyeongju City (N35°.39), and the inland distribution limits were Uljin-gun (N°.’.. E°.’). Speaking of islands, Uleungdo was the distribution limit.
2) The fruit-bearing limits were Taean-gun of the West Coast (N 36.40) and Nonsan-gun, Boseong-gun and Gijang-gun of Chungcheong-namdo of the inland, and Gyeongju City (N35°.39) and Uljin-gun (N°.’.. E°.’) of the East Coast. Though some regions were only partially confirmed, however, the best region for cultivation is thought to be from Yeonggwang-gun, Jeolla-bukdo to Gijang-gun, Busan City including the Southern Coast.
3) The DPPH scavenging measurement was used to detect the antioxidant activity of the leaf extracts and fractions of Hedera rhombea (Miq.) Bean. Radical scavenging activity was confirmed in the 80% ethanol extract, and the ethyl acetate and butanol fraction. Also, the xanthine oxidase depression effect was confirmed in the 에틸아세테티즈 fraction, superoxide whereas the scavenging activity was confirmed in the 80% ethanol extract, and the dichloromethane, ethyl acetate and butanol fraction respectively.
4) RAW 264.7 cell was stimulated with LPS, and extracts and fractions were treated. NO, iNOS, COX-2, PGE2 depression effect was confirmed in the hexane and dichloromethane fraction. However, the depression effect found in the hexane fraction is thought to be attributed to the inhibitory effect of cytotoxicity.
5) The extracts and fractions were treated in the myelogenous leukemia, colorectal cancer and hepatoma cells, and the MTT assay was used to confirm the cancel cell growth inhibition effect. The cell growth inhibition effect was in the hexane, dichloromethane and ethyl acetate fractions.
6) The dichloromethane fraction from Hedera rhombea (Miq.) Bean was treated in the HL-60. The sub-G1 hypodiploid cells increased due to the induction of apoptosis in the cell cycle, and the apoptotic bodies were confirmed in the process of apoptosis. Also, in protein expression, the generation of BCL-xL, a factor related to anti-apoptosis, was depressed, and Bax, caspase-3, caspase-9 and PARP, factors related to apoptosis, were activated. 3. Comparison of the documentary records on Hedera rhombea (Miq.) Bean of Jeolla-namdo and the result of on-site investigation |
