성체줄기세포 번역(한국어 원본)인체 상악동점막에는 조골세포로 분화할 수 있는 성체줄기세포가 존재하며, 이는 골이식술 시행시 신생골형성에 기여한다. 본 연구는 상악동점막에서 유래한 성체줄기세포에 골형성단백질-2 (bone morphogenetic protein-2, BMP-2) 또는 심바스태틴 (simvastatin)을 적용하였을 때, 신생골 형성을 증진시킬 수 있는지 알아보고자 시행하였다.
16마리의 Spraque-Dawley (SD) 흰쥐를 4군으로 분류하고, 두개골에 직경 5 mm 결손부를 만들었다. BMP군에는 BMP-2가 첨가된 상악동점막 줄기세포 (sinus membrane stem cells)와 전달체 역할을 하는 베타-삼인산칼슘염 (β-tricalcium phosphate, β-TCP)을 조합하여 결손부를 채웠고, 심바스태틴군은 심바스태틴이 첨가된 상악동점막 줄기세포와 β-TCP를 혼합하여 결손부를 채웠으며, 상악동점막 줄기세포군은 상악동점막 줄기세포와 β-TCP를 채웠고, 대조군은 β-TCP만으로 결손부를 채웠다. 이식수술 4, 8주 경과 후에 각 군당 2마리씩을 희생한 후 조직학적 검사와 분자생물학적검사 (reverse transcriptase-polymerase chain reaction, RT-PCR)를 시행하였다.
조직학적 소견상 4주와 8주 모두에서 BMP군과 심바스태틴군에서의 신생골형성이 상악동점막 줄기세포군 및 대조군보다 많이 관찰되었다. 또한 RT-PCR 결과 Ⅰ형 교원질 (collagen type Ⅰ) mRNA는 4주, 8주 모두 BMP군과 심바스태틴군에서 높게 발현되었으며 오스테오칼신 (osteocalcin) mRNA는 4주에서는 BMP군만 높게 발현되었으나 8주에서는 BMP군과 심바스태틴군 모두 높게 발현되었다.
이상과 같은 결과로 골형성단백질-2와 심바스태틴은 상악동점막 유래 성체줄기세포의 골형성능을 촉진시킬 수 있는 것으로 추론되며, 임상적으로 적용할 때, 바람직한 골형성 효과를 기대할 수 있을 것으로 사료된다. |
성체줄기세포 번역(영어 번역본)The human maxillary sinus membrane contains adult stem cells, which have the potential to differentiate into osteoblast. The purpose of this study was to evaluate whether new bone formation can be accelerated when bone morphogenetic protein-2, BMP-2 or simvastatin is applied on the adult stem cells.
16 Spraque-Dawley (SD) albino rats were divided into four groups and 5 mm diameter defect was created in the crania of these subjects.
The defects were filled with β-tricalcium phosphate (β-TCP) that is combined with BMP-2 enhanced sinus membrane stem cells (SMSCs) for the BMP group. The defects were filled with β-TCP that is combined with simvastatin enhanced SMSCs for the simvastatin group. The defects were filled with β-TCP that is mixed with SMSCs for SMSC group. The defects were filled with just β-TCP for the control group. Two rats of each group were killed 4 and 8 weeks after the surgery for histologic staining, after which reverse transcriptase-polymerase chain reaction (RT-PCR) was carried out.
It was observed from the histologic results that in both 4th week and the 8th week that simvastatin and BMP groups showed much more bone regeneration compared to control and sinus membrane stem cells (SMSCs) groups. In addition, RT-PCR analysis revealed that collagen type I mRNAs was higher in simvastatin and BMP groups than control and SMSC groups in both 4th and 8th week. The expression of Osteocalin mRNA was high in the 4th week for just the BMP group, but was high in both BMP and simvastatin group in the 8th week.
The results suggest that bone morphogenetic protein-2 and simvastatin can accelerate the maxillary sinus membrane derived adult stem cells’ ability in bone formation and when clinically applied, it is thought that effective bone formation can be expected. |
